The Genomic and RNA Profiling core offers various RNA-seq chemistries for many sample types.
Total RNA-seq: Capture of both coding and non-coding RNAs. Compatible with degraded RNA. Full-length transcripts are captured. Recommended read depth is around 50-100 million reads/sample although deeper sequencing may be required in some cases.
Low-Input Total RNA-seq: Capture of both coding and non-coding RNAs. Compatbile with inputs down to 250 pg of RNA. Recommended for RNA-seq of cfRNA, rare cell populations or FACS-sorted cells.
PolyA-RNA-seq: Capture of coding RNAs. Requires intact RNA. Full-length transcripts are captured. Recommended read depth is 15-30 million reads/sample although deeper sequencing may be required in some cases.
3’ RNA-seq: Capture of coding RNAs. cDNA is generated from 3’end of transcript. Compatible with degraded RNA. Recommended read depth is 10-20 million reads/sample.
Micro-RNA-seq: Enables miRNA profiling and discovery with cfRNA and cellular RNA samples. Required read depth is 5-20 million reads/sample.
The Genomic and RNA Profiling core in partnership with the Human Tissue Acquisition and Pathology Core offers the 10X Genomics Visium technology for in situ spatial profiling of fresh-frozen and FFPE tissue samples. For more information, please visit the 10X website.
Chromatin immunoprecipitation sequencing allows for genome-wide profiling of protein-DNA interactions and of histone PTMs. GARP offers ChIP-seq library construction starting with ChIP and Input DNA.
Whole-Genome Bisulfite Sequencing (WGBS) allows for genome-wide DNA methylation (5-mC) profiling. The Genomic and RNA Profiling core offers a one-stop WGBS workflow starting with DNA fragmentation, bisulfite conversion, library preparation and ending with sequencing.
By focusing on sequencing the protein-coding regions of the genome, researchers can identify disease-causing variants. At GARP, we offer library construction with the Twist Biosciences Human and Mouse Exome Panels. For panel content information, please visit the Twist website.