Cell and Gene Therapy for Musculoskeletal Disorders



Publications 2016 - 2018


Gugala Z, Olmsted-Davis EA, Xiong Y, Davis EL, Davis AR. Trauma-Induced Heterotopic Ossification Regulates the Blood-Nerve Barrier. Front Neurol. 2018 Jun 5;9:408. doi: 10.3389/fneur.2018.00408. eCollection 2018. Review. PMID: 29922221

Davis EL, Davis AR, Gugala Z, Olmsted-Davis EA. Is heterotopic ossification getting nervous?: The role of the peripheral nervous system in heterotopic ossification. Bone. 2018 Apr;109:22-27. doi: 10.1016/j.bone.2017.07.016. Epub 2017 Jul 15. Review. PMID: 28716552

Salisbury EA, Dickerson AR, Davis TA, Forsberg JA, Davis AR, Olmsted-Davis EA. Characterization of Brown Adipose-Like Tissue in Trauma-Induced Heterotopic Ossification in Humans. Am J Pathol. 2017 Sep;187(9):2071-2079. doi: 10.1016/j.ajpath.2017.05.012. Epub 2017 Jul 4 PMID: 28686851

Alvarez-Urena P, Zhu B, Henslee G, Sonnet C, Davis E, Sevick-Muraca E, Davis A, Olmsted-Davis E. J Development of a Cell-Based Gene Therapy Approach to Selectively Turn Off Bone Formation. Cell Biochem. 2017 Nov;118(11):3627-3634. doi: 10.1002/jcb.26220. Epub 2017 Jul 31.

Olmsted-Davis EA, Salisbury EA, Hoang D, Davis EL, Lazard Z, Sonnet C, Davis TA, Forsberg JA, Davis AR. Progenitors in Peripheral Nerves Launch Heterotopic Ossification. Stem Cells Transl Med. 2017 Apr;6(4):1109-1119.

Alvarez-Urena P, Davis E, Sonnet C, Henslee G, Gugala Z, Strecker EV, Linscheid LJ, Cuchiara M, West J, Davis A, Olmsted-Davis E. Encapsulation of Adenovirus BMP2-Transduced Cells with PEGDA Hydrogels Allows Bone Formation in the Presence of Immune Response. Tissue Eng Part A. 2017 Mar;23(5-6):177-184.

Davis EL, Sonnet C, Lazard ZW, Henslee G, Gugala Z, Salisbury EA, Strecker EV, Davis TA, Forsberg JA, Davis AR, Olmsted-Davis EA. Location-dependent heterotopic ossification in the rat model: The role of activated matrix metalloproteinase 9. J Orthop Res. 2016 Nov;34(11):1894-1904.

Davis EL, Salisbury EA, Olmsted-Davis E, Davis AR. Anaplerotic Accumulation of Tricarboxylic Acid Cycle Intermediates as Well as Changes in Other Key Metabolites During Heterotopic Ossification. J Cell Biochem. 2016 Apr;117(4):1044-53.


Publications 2011 - 2015


Lazard ZW, Olmsted-Davis EA, Salisbury EA, Gugala Z, Sonnet C, Davis EL, Beal E 2nd, Ubogu EE, Davis AR. Osteoblasts Have a Neural Origin in Heterotopic Ossification. Clin Orthop Relat Res. 2015 Sep;473(9):2790-806.

Reichel LM, Salisbury E, Moustoukas MJ, Davis AR, Olmsted-Davis E Molecular mechanisms of heterotopic ossification. J Hand Surg Am. 2014 Mar;39(3):563-6. Review. No abstract available.Lu Y, Darne CD, Tan IC, Zhu B, Hall MA, Lazard ZW, Davis AR, Simpson L, Sevick-Muraca EM, Olmsted-Davis EA. Far-red fluorescence gene reporter tomography for determination of placement and viability of cell-based gene therapies. Opt Express. 2013 Oct 7;21(20):24129-38.

Sonnet C, Simpson CL, Olabisi RM, Sullivan K, Lazard Z, Gugala Z, Peroni JF, Weh JM, Davis AR, West JL, Olmsted-Davis EA. Rapid healing of femoral defects in rats with low dose sustained BMP2 expression from PEGDA hydrogel microspheres. J Orthop Res. 2013 Oct;31(10):1597-604.

Salisbury EA, Lazard ZW, Ubogu EE, Davis AR, Olmsted-Davis EA. Transient brown adipocyte-like cells derive from peripheral nerve progenitors in response to bone morphogenetic protein 2. Stem Cells Transl Med. 2012 Dec;1(12):874-85.

Stephens EH, Saltarrelli JG Jr, Balaoing LR, Baggett LS, Nandi I, Anderson KM, Morrisett JD, Reardon MJ, Simpson MA, Weigel PH, Olmsted-Davis EA, Davis AR, Grande-Allen KJ. Hyaluronan turnover and hypoxic brown adipocytic differentiation are co-localized with ossification in calcified human aortic valves. Pathol Res Pract. 2012 Nov 15;208(11):642-50.

Salisbury E, Hipp J, Olmsted-Davis EA, Davis AR, Heggeness MH, Gannon FH. Histologic identification of brown adipose and peripheral nerve involvement in human atherosclerotic vessels. Hum Pathol. 2012 Dec;43(12):2213-22.

Mumaw J, Jordan ET, Sonnet C, Olabisi RM, Olmsted-Davis EA, Davis AR, Peroni JF, West JL, West F, Lu Y, Stice SL. Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs. Int J Biomater. 2012;2012:861794.

Salisbury EA, Olmsted-Davis EA, Davis AR. Adverse events and bone morphogenetic protein-2. Spine J. 2011 Aug;11(8):802.

Salisbury E, Rodenberg E, Sonnet C, Hipp J, Gannon FH, Vadakkan TJ, Dickinson ME, Olmsted-Davis EA, Davis AR. Sensory nerve induced inflammation contributes to heterotopic ossification. J Cell Biochem. 2011 Oct;112(10):2748-58.

Azhdarinia A, Wilganowski N, Robinson H, Ghosh P, Kwon S, Lazard ZW, Davis AR, Olmsted-Davis E, Sevick-Muraca EM. Characterization of chemical, radiochemical and optical properties of a dual-labeled MMP-9 targeting peptide. Bioorg Med Chem. 2011 Jun 15;19(12):3769-76.

Rodenberg E, Azhdarinia A, Lazard ZW, Hall M, Kwon SK, Wilganowski N, Salisbury EA, Merched-Sauvage M, Olmsted-Davis EA, Sevick-Muraca EM, Davis AR. Matrix metalloproteinase-9 is a diagnostic marker of heterotopic ossification in a murine model. Tissue Eng Part A. 2011 Oct;17(19-20):2787-96.

Hsu CW, Olabisi RM, Olmsted-Davis EA, Davis AR, West JL. Cathepsin K-sensitive poly(ethylene glycol) hydrogels for degradation in response to bone resorption. J Biomed Mater Res A. 2011 Jul;98(1):53-62.

Lazard ZW, Heggeness MH, Hipp JA, Sonnet C, Fuentes AS, Nistal RP, Davis AR, Olabisi RM, West JL, Olmsted-Davis EA. Cell-based gene therapy for repair of critical size defects in the rat fibula. J Cell Biochem. 2011 Jun;112(6):1563-71.

Olabisi RM, Lazard Z, Heggeness MH, Moran KM, Hipp JA, Dewan AK, Davis AR, West JL, Olmsted-Davis EA. An injectable method for noninvasive spine fusion. Spine J. 2011 Jun;11(6):545-56.

Stephens EH, Saltarrelli JG, Baggett LS, Nandi I, Kuo JJ, Davis AR, Olmsted-Davis EA, Reardon MJ, Morrisett JD, Grande-Allen KJ. Differential proteoglycan and hyaluronan distribution in calcified aortic valves. Cardiovasc Pathol. 2011;20(6);334-42.

Liu SH, Smyth-Templeton N, Davis AR, Davis EA, Ballian N, Li M, Liu H, Fisher W, Brunicardi FC. Multiple treatment cycles of liposome-encapsulated adenoviral RIP-TK gene therapy effectively ablate human pancreatic cancer cells in SCID mice. Surgery. 2011 Apr;149(4):484-95.


Publications 2006 - 2010


Salisbury E, Sonnet C, Heggeness M, Davis AR, Olmsted-Davis E. Heterotopic ossification has some nerve. Crit Rev Eukaryot Gene Expr. 2010;20(4):312-24. Review.

Dewan AK, Dewan RA, Calderon N, Fuentes A, Lazard Z, Davis AR, Heggeness M, Hipp JA, Olmsted-Davis EA. Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model. J Orthop Surg Res. 2010 Aug 21;5:58.

Olabisi RM, Lazard ZW, Franco CL, Hall MA, Kwon SK, Sevick-Muraca EM, Hipp JA, Davis AR, Olmsted-Davis EA, West JL. Hydrogel microsphere encapsulation of a cell-based gene therapy system increases cell survival of injected cells, transgene expression, and bone volume in a model of heterotopic ossification. Tissue Eng Part A. 2010 Dec;16(12):3727-36.

Dilling CF, Wada AM, Lazard ZW, Salisbury EA, Gannon FH, Vadakkan TJ, Gao L, Hirschi K, Dickinson ME, Davis AR, Olmsted-Davis EA. Vessel formation is induced prior to the appearance of cartilage in BMP-2-mediated heterotopic ossification. J Bone Miner Res. 2010 May;25(5):1147-56.

Fouletier-Dilling CM, Gannon FH, Olmsted-Davis EA, Lazard Z, Heggeness MH, Shafer JA, Hipp JA, Davis AR. Efficient and rapid osteoinduction in an immune-competent host. Hum Gene Ther. 2007 Aug;18(8):733-45.

Gugala Z, Davis AR, Fouletier-Dilling CM, Gannon FH, Lindsey RW, Olmsted-Davis EA. Adenovirus BMP2-induced osteogenesis in combination with collagen carriers. Biomaterials. 2007 Oct;28(30):4469-79.

Bikram M, Fouletier-Dilling C, Hipp JA, Gannon F, Davis AR, Olmsted-Davis EA, West JL. Endochondral bone formation from hydrogel carriers loaded with BMP2-transduced cells. Ann Biomed Eng. 2007 May;35(5):796-807.

Shafer J, Davis AR, Gannon FH, Fouletier-Dilling CM, Lazard Z, Moran K, Gugala Z, Ozen M, Ittmann M, Heggeness MH, Olmsted-Davis E. Oxygen tension directs chondrogenic differentiation of myelo-monocytic progenitors during endochondral bone formation. Tissue Eng. 2007 Aug;13(8):2011-9.

Olmsted-Davis E, Gannon FH, Ozen M, Ittmann MM, Gugala Z, Hipp JA, Moran KM, Fouletier-Dilling CM, Schumara-Martin S, Lindsey RW, Heggeness MH, Brenner MK, Davis AR. Hypoxic adipocytes pattern early heterotopic bone formation. Am J Pathol. 2007 Feb;170(2):620-32. Bosch P, Fouletier-Dilling C, Olmsted-Davis EA, Davis AR, Stice SL. Efficient adenoviral-mediated gene delivery into porcine mesenchymal stem cells. Mol Reprod Dev. 2006 Nov;73(11):1393-403.

Wang X, Olmsted-Davis E, Davis A, Liu S, Li Z, Yang J, Brunicardi FC. Specific targeting of pancreatic islet cells in vivo by insulin-promoter-driven adenoviral conjugated reporter genes. World J Surg. 2006 Aug;30(8):1543-52.

Xue W, Wallin R, Olmsted-Davis EA, Borrás T. Matrix GLA protein function in human trabecular meshwork cells: inhibition of BMP2-induced calcification process. Invest Ophthalmol Vis Sci. 2006 Mar;47(3):997-1007.


Publications 2001 - 2005


Fouletier-Dilling CM, Bosch P, Davis AR, Shafer JA, Stice SL, Gugala Z, Gannon FH, Olmsted-Davis EA. Novel compound enables high-level adenovirus transduction in the absence of an adenovirus-specific receptor. Hum Gene Ther. 2005 Nov;16(11):1287-97.

Dacquin R, Mee PJ, Kawaguchi J, Olmsted-Davis EA, Gallagher JA, Nichols J, Lee K, Karsenty G, Smith A. Knock-in of nuclear localised beta-galactosidase reveals that the tyrosine phosphatase Ptprv is specifically expressed in cells of the bone collar. Dev Dyn. 2004 Apr;229(4):826-34.

Yotnda P, Davis AR, Hicks MJ, Templeton NS, Brenner MK. Liposomal enhancement of the antitumor activity of conditionally replication-competent adenoviral plasmids. Mol Ther. 2004 Apr;9(4);489-95.

Bollard CM, Straathof KC, Huls MH, Leen A, Lacuesta K, Davis A, Gottschalk S, Brenner MK, Heslop HE, Rooney CM. The generation and characterization of LMP2-specific CTLs for use as adoptive transfer from patients with relapsed EBV-positive Hodgkin disease. J Immunother. 2004 Jul-Aug;27(4):317-27.

Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR. Primitive adult hematopoietic stem cells can function as osteoblast precursors. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26): 15877-82.

Gugala Z, Olmsted-Davis EA, Gannon FH, Lindsey RW, Davis AR. Osteoinduction by ex vivo adenovirus-mediated BMP2 delivery is independent of cell type. Gene Ther. 2003 Aug;10(16):1289-96.

Olmsted EA, Kaplan FS, Shore EM. Bone morphogenetic protein-4 regulation in fibrodysplasia ossificans progressiva. Clin Orthop Relat Res. 2003 Mar;(408):331-43.

Sili U, Huls MH, Davis AR, Gottschalk S, Brenner MK, Heslop HE, Rooney CM Large-scale expansion of dendritic cell-primed polyclonal human cytotoxic T-lymphocyte lines using lymphoblastoid cell lines for adoptive immunotherapy. J Immunother. 2003 May-Jun;26(3):241-56.

Gottschalk S, Edwards OL, Sili U, Huls MH, Goltsova T, Davis AR, Heslop HE, Rooney CM. Generating CTLs against the subdominant Epstein-Barr virus LMP1 antigen for the adoptive immunotherapy of EBV-associated malignancies. Blood. 2003 Mar 101(5):1905-12.

Olmsted-Davis EA, Gugala Z, Gannon FH, Yotnda P, McAlhany RE, Lindsey RW, Davis AR. Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Hum Gene Ther. 2002 Jul 20;13(11):1337-47.

Yotnda P, Chen DH, Chiu W, Piedra PA, Davis A, Templeton NS, Brenner MK Bilamellar cationic liposomes protect adenovectors from preexisting humoral immune responses. Mol Ther. 2002 Mar;5(3);233-41.

Olmsted EA, Blum JS, Rill D, Yotnda P, Gugala Z, Lindsey RW, Davis AR. Adenovirus-mediated BMP2 expression in human bone marrow stromal cells. J Cell Biochem. 2001 Apr 2-27;82(1):11-21.

Yotnda P, Onishi H, Heslop HE, Shayakhmetov D, Lieber A, Brenner M, Davis A. Efficient infection of primitive hematopoietic stem cells by modified adenovirus. Gene Ther. 2001 Jun;8(12):930-7.


Contact Us


Alan R. Davis
(713) 798-1237

Elizabeth A. Olmsted-Davis
(713) 798-1253

Lab Phone: (713) 798-1238
Lab Fax: (713) 798-1230