Active Protocols
PCR amplification for 5xFAD model
Mouse biopsy DNA extraction
Taq polymerase preparation
PCR amplification for TTA and APP (CaMKIIα-TTA and most other TTA lines using original TTA sequence)
PCR amplification for TTA2 and mTTA (Nop-TTA, R26-LNL-TTA, and other lines using TTA2S sequence)
PCR amplification for total Cre and CreER (original and improved Cre and CreER, Nestin-creER, DAT-cre, POMC-cre, CaMK2a-CreER and most other Cre constructs)
PCR amplification for GFP/YFP (Ai3 cre reporter, flop-GluCl/GlyCl, TRE3G-GlyCl, and most other GFP/YFP constructs)
PCR amplification for tdTomato (Ai14 cre reporter)
PCR amplification for mCherry (TRE-Kir-mCherry)
PCR amplification for floxed STOP (loxP-PGKneo primers, used for confirmation of flop-GluCl/GlyCl
PCR amplification for ROSA26-LSL-APP (Cre-inducible APP)
PCR amplification for ROSA26-LSL-APP homozygous vs heterozygous (Cre-inducible APP)
PCR amplification for human MAPT (Prp-MAPTP301S PS19)
PCR amplification for CHRM4 (TetO-CHRM4 DREADDi)
qPCR amplification for TTA2 and CHRM4 (Nop-TTA and tetO-CHRM4)
Archived Protocols
PCR amplification for original Cre and CreER
PCR amplification for tdTomato (Ai9 cre reporter)
PCR amplification for LacZ (TTA and cre reporters)
PCR amplification for floxed-stop TTA converter (zTTA)
PCR amplification for YFP-WPRE (Ai3 cre reporter and flop-GluCl)
PCR amplification for YFP-WPRE (long extension time for TRE3G-GlyCl and flop-GlyCl)
PCR amplification for GFP/YFP and WPRE (archived Jan 2019)
PCR amplification for CD11b-HSVTK
PCR amplification for SOD1
PCR amplification for iDTR
PCR amplification for H2B-GFP TTA reporter
PCR amplification for hTau
PCR amplification for mTau KO
PCR amplification for Pitx3-TTA
PCR amplification for floxed APP
PCR amplification for APP / PS1
PCR amplification for PS1 alone
