USDA/ARS Children's Nutrition Research Center

Confocal Microscopy and Image Analysis Core Instrumentation

Master
Content

Olympus Fluoview confocal microscopy system consisting of an IX70 microscope, four lasers (blue diode, argon, green HeNe, and red HeNe) a laser combiner and FV300 confocal scanner, a bright-field detector, a Spot RT color CCD camera, a computer work station, and a Kodak 8600 dye-sublimation printer

Olympus IX70 microscope equipped with Narashigi micromanipulators and an Eppendorf microinjection system

Zeiss Axiophot microscope equipped for epifluoresence with an Optronix color CCD camera

Nikon SMZ1500 stereo-zoom microscope equipped for both bright-field and fluorescent work linked to a Nikon color CCD camera

Thermo-Shandon Cryotome equipped with an Instrumedics tape transfer device

Leica microtome

Image analysis workstation that consists of a DELL Optiplex GX620 processor with a Radeon X600SE video card, Adobe Photoshop image processing software and Image-Pro Plus image processing and analysis software. The image analysis software consists of a basic analysis module with plug-in modules for image deconvolution (Sharp Stack Plus Ver 5.0), and 3 dimensional reconstruction (3D Constructor).

Questions

Questions concerning suitability of the system for particular applications can be directed to Dr. Darryl Hadsell, associate professor Pediatrics and Molecular and Cellular Biology.