GTPases play an important role in the assembly of ribosomes in all three kingdoms of life. The molecular mechanisms by which they function are largely unknown. We are studying the ribosome assembly GTPase RbgA in Bacillus subtilis in an attempt to understand how these proteins act in the maturation of the large ribosomal subunit using a combination of biochemical, structural and genetic approaches. Interestingly, mutation or depletion of RbgA results in the accumulation of a 45S ribosome assembly intermediate that is arrested at a very late stage of development. Work on eukaryotic homologs of RbgA suggests that these proteins are involved in a late assembly step of the large ribosomal subunit. The results from this bacterial work will have important implications for the formation of cytoplasmic, mitochondrial, and chloroplast ribosomes.
Razi A, Britton RA, Ortega J. (2017) The impact of recent improvements in cryo-electron microscopy technology on the understanding of bacterial ribosome assembly. Nucleic Acids Res. 45(3):1027-1040.
Ni X, Davis JH, Jain N, Razi A, Benlekbir S, McArthur AG, Rubinstein JL, Britton RA, Williamson JR, Ortega J. (2016) YphC and YsxC GTPases assist the maturation of the central protuberance, GTPase associated region and functional core of the 50S ribosomal subunit. Nucleic Acids Res. 44(17):8442-55.
Gulati M, Jain N, Davis JH, Williamson JR, Britton RA (2014). Functional interaction between ribosomal protein L6 and RbgA during ribosome assembly. PLoS Genet. 10(10).
Jomaa A, Jain N, Davis JH, Williamson JR, Britton RA, Ortega J. (2014). Functional domains of the 50S subunit mature late in the assembly process. Nucleic Acids Res. 42(5):3419-35.
Gulati M, Jain N, Anand B, Prakash B, Britton RA (2013). Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation. Nucleic Acids Res. 41(5):3217-27
Achila D, Gulati M, Jain N, Britton RA. (2012). Biochemical characterization of the ribosome assembly GTPase RbgA in Bacillus subtilis. Journal of Biological Chemistry. 153:564-73.
Britton, RA. Role of GTPases in ribosome assembly. (2009). Annual Review of Microbiology. 2009;63:155-76.
Uicker, WC, Schaefer, L, Koenigsknecht, M, and Britton, RA (2007). The essential GTPase YqeH is required for proper ribosome assembly in Bacillus subtilis. Journal of Bacteriology. 189:2926-29.
Schaefer, L, Uicker, WU, Wicker-Planquart, C, Foucher, AE, Jault JM and Britton, RA (2006). Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis. Journal of Bacteriology. 188:8252-8258.
Uicker, WU, Schaefer, L and Britton, RA (2006). The essential GTPase RbgA (YlqF) is required for 50S ribosome assembly in Bacillus subtilis. Molecular Microbiology. 59:528-40.