Research

Bone Histology/Immunohistochemistry Core

Master
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About the Core

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This core facility provides high quality histological services and is available to assist investigators in the bone disease program within Texas Medical Center, as well as, researchers working with animal bone-related tissues as a collaborative resource.

Our facility utilizes a Leica CM3050S cryostat attached with Cryo-Jane Tape Transfer system. The Cryo-Jane Tape-Transfer system is designed to produce paraffin-quality frozen sections as thin as two microns that are wrinkle-free, uncompressed, fully intact and bonded to the microscope slide in less than three minutes. This process makes use of snap-frozen tissue to minimize damage by ice crystals and preserves virtually all fine structure present in the snap-frozen block of tissue. Water-soluble enzymes, antigens and labeling proteins (GFP) are also preserved in-situ, and with appropriate fixation and staining, true localization of enzyme and antigen activity can be visualized. This equipment is especially useful for preparing frozen sections of fat, bone marrow, undecalcified bone and skin samples.

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Core Services

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  • Processing and sectioning of paraffin-embedded specimens
  • Processing and sectioning of frozen tissues (in OCT)
  • Cryo-Jane Tape transfer sectioning
  • H&E etc routing staining for bone and cartilage
  • Immunohistochemical / immunofluorescence staining
  • Consultation and Staining for immunohistochemistry
  • Training investigators and staff
  • Consultation with histology personnel
  • Protocol development for tissue collection and processing
  • TUNEL Assay
  • Assistance with FISH/ISH assays
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Request a Service

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Contact our core staff to request for one of the services listed above.

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Core Director

People
First Name
Yangjin
Last Name
Bae
Assistant Professor
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Core Staff

People
First Name
Ming-Ming
Last Name
Jiang
Dir, Laboratory, Asst
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